The life cycle and postembryonic development of Tityus confluens Borelli, 1899 and Tityus ocelote (Francke & Stockwell, 1987) are for the first time described. The duration of embryonic development was estimated to range from 86 to 184 (N = 11) days in T. confluens and 68 to 72 (N = 4) days in T. ocelote. The molts between juvenile instars and adult stage took place at average ages of: 5, 38, 106, 157 days in T. confluens and 4, 60, 62, 97 days in T. ocelote. Compared with other species of the family Buthidae in the same range of size this data are similar. The morphometric growth factor (Dyar’s constant) are higher than those observed for other scorpions of the genus Tityus of the same size. Parthenogenesis is confirmed for T. confluens based on captive breed females raised in isolation since offspring (N = 156). No males were observed over a period of 3 generations.